After going their separate ways, Scooby-Doo, Shaggy, Velma, Daphne, and Fred reunite to investigate the ghost of Moonscar the pirate on a haunted bayou island, but it turns out the swashbuckler's spirit isn't the only creepy character on the island. The sleuths also meet up with cat creatures and zombies... and it looks like for the first time in their lives, these ghouls might actually be real.

Scooby-Doo and his pals win an all-expense paid vacation and embark on a trip of a lifetime to a tropical paradise. Their destination however, turns out to be Zombie Island. As soon as they arrive, they realize the place looks strangely familiar and is reminiscent of a trip they took years ago, in which they became wrapped up in a mystery involving zombies. The gang soon learns that their trip to paradise comes with a price when the zombies re-emerge and attack their hotel. Will Scooby-Doo and the Mystery Inc. gang finally solve the mystery behind Zombie Island?